Introduction to Plant Tissue Culture

In March 2013, we are lucky enough thanks to our University that providing us an opportunity to have a fieldwork laboratory experience on the subject "Cell Tissue Culture". To think that we actually able to conduct an experiment that what we learned in class is excited enough and wanted to share this joy with our fellow readers.

Some of us observing the process of seed culturing in laminar flow

Ryan doing the sterilization, bear in mind that its the crucial step before we conduct any experiment

This is to prevent any contamination to our sample

Proudly presenting our seed sample

In this lab experiment, we didn't just practice tissue culture

We got ourself a ticket to visit each part of the lab (we don't get to see this outside easily) 

Such as culture room (picture shown above), media preparation room, fermentation lab and molecular lab

Some callus sample in culture room

Callus is the dedifferentiated plant cells, we will further explain it in details in the up coming post

As for now, callus can be explained as picture below:

Callus is originate a small part of mature plant, isolated and culture with medium to induce callus formation

It is very important in plant biotechnology as it will be used as a research subject in a variety ways of plant transformation studies

The result of the seed culture: shoots that grow in-vitro

It's magical moment, I know hahahah

We got ourself some lab practice too in lab practical 5: preparing media

Some expansive nutrient in powder form

Excellent lab practice with caution is the key to successful conduct an experiment with less error and expected results.

Until then, will update more in details in the next post :)