Seed culturing

Here we will be presenting what we have experienced in lab experiment of seed culturing. Well in short, seed culturing is growing the seed in-vitro by supplying everything necessary for the seed to become plant.

For your information, in-vitro is a mean of growing the sample in a test tube, culture dish, or elswhere outside the living organism. 

On other hand, by the mean of supplying everything necessary for the seed is providing nutrient requires or medium as well as other factors such as pH, temperature, mosture etc. 

But first, before we proceed to seed culturing, we need to prepare a medium (home for the seed to grow)

There are quite a few number of common medium used but to make it simple for our reader, we used MS medium because it can be use to grow general plant sample. 

How to make MS medium:

We can make MS medium by mixing Gamborg's B5 vitamin, FeNaEDTA, macronutrient and micronutrient.

However, we have to prepare this ourself

Example of the micronutrient preparation

We must weight accordingly 

Stir well by using magnetic stirrer

Upon finish, we put it into a bottle

These are the prepared component to make MS medium

We measure every volume of the component require and mix it into a beaker

Adjust the pH to 6

This is the agar powder for making the medium "hard" for the seed incubation

But first we must melt the powder by putting it into oven

Pour into the prepared flasks

Seal properly to avoid contamination

This is how the MS medium looks like

FOR MORE DETAILS ON MS MEDIUM PREPARATION PLEASE REFER TO OUR VIDEO

We can proceed to the seed culturing now

Surface sterilization of the seed samples

This is how it looks like after air dry

Carefully put the seed samples into the MS medium in a sterile condition

Seal with aluminium fold and culture it in culture room

However this is the simplified way of presenting how we conduct seed culturing

FOR MORE DETAILS IN SEED CULTURING AND THE RESULT OF SEED CULTURING PLEASE REFER TO OUR VIDEO.